Vilon (50mg)

What is Vilon (50 mg)?

Vilon (50 mg) is a synthetic dipeptide made from two amino acids: L-lysine and L-glutamic acid (Lys–Glu). It is commonly grouped with immune and thymic peptide research due to its association with thymic tissue extracts, which have been studied for its potential role in immune regulation.

In laboratory research, Vilon is used to study how very small peptides may influence chromatin structure, gene expression, and immune cell function—particularly in models related to aging, immune regulation, and thymus-dependent immune processes.

Product Specifications

• CAS Number: 45234-02-4
• Peptide Sequence: Lys-Glu (L-Lys-L-Glu)
• Chemical Formula: C₁₁H₂₁N₃O₅
• Molecular Weight: 275.30 g/mol
• Purity: ≥99% (research grade; HPLC/MS verified)
• Packaging Format: 50 mg lyophilized powder in a sealed research-grade vial
• Storage Conditions (unreconstituted): Store at −20 °C, protect from light, keep vial tightly closed
• Intended Use: For laboratory research use only; not for human or veterinary use

Key Characteristics of Vilon (50 mg)

• Thymus-Derived Bioregulator: Vilon is a synthetic analogue of a peptide associated with thymic tissue extracts and is often studied in relation to immune and thymic functions.
• Chromatin and Gene Expression: In cultured lymphocytes from older donors, Vilon has been reported to promote chromatin decondensation (the “reactivation” of DNA) and increase transcription in previously inactive regions, such as ribosomal DNA. This effect is often considered an epigenetic-like process in mechanistic studies.
• Immune Cell Function: In vitro and ex vivo studies have shown that Vilon can influence cytokine gene expression, including interleukin-2 (IL-2) in splenocytes, and can affect cell proliferation in thymus cell cultures.
• Aging and Preclinical Observations: In rodent studies, Vilon has been associated with changes in spontaneous tumor growth and lifespan, providing insights into aging biology and immune surveillance. These findings are preclinical and do not imply clinical outcomes.
• Transcriptomic and Tissue Profiling: Microarray-based studies in mouse heart and other tissues have shown that Vilon exposure can lead to broad transcriptional shifts, particularly in genes related to the extracellular matrix, microvasculature, and stress response pathways.
• High-Purity, Verified Material: Vilon is supplied as a ≥99% purity lyophilized peptide, verified by HPLC/MS, ensuring reliable use across biochemical, cellular, and animal research workflows.

How Vilon (50 mg) Supports Research

Vilon (50 mg) is used as a research tool to explore the intersection of epigenetic regulation and thymus-linked immune biology. In lymphocyte and thymus-cell systems, it is applied to investigate how short peptides may affect:

• Chromatin compaction and DNA accessibility
• Transcription patterns, including cytokine genes such as interleukin-2 (IL-2)
• Immune activation-related gene programs in controlled culture settings

In animal studies, Vilon has been used to investigate age-related gene expression drift, spontaneous tumor dynamics, gastrointestinal enzyme activity, and microvascular signaling. These findings help generate hypotheses in aging and immune regulation research, but do not imply therapeutic use.

Research Applications & Usage Information

Vilon (50 mg) is commonly used in a variety of mechanistic and preclinical research designs, including:

• Epigenetics and Chromatin Biology: Studying chromatin accessibility (euchromatin vs. heterochromatin), nucleolar activity, and transcriptional changes in lymphocytes from young vs. older donors.
• Immune-Cell and Cytokine Gene Regulation: Measuring IL-2 expression and related cytokine transcripts in splenocyte or thymus-derived cultures, along with cell activation and proliferation markers.
• Thymus and Lymphoid Tissue Models: Investigating thymus-cell culture responses, including shifts in cell populations and proliferation markers, to explore thymic signaling in vitro.
• Tumor Biology and Immune Surveillance (Preclinical): Studying associations with spontaneous tumor growth rates, survival metrics, and gene expression in rodent tumor models, particularly related to immune surveillance.
• Aging-Related Gastrointestinal and Cardiovascular Research: Exploring enzyme activity and transport in gastrointestinal tissues from aging animals, along with transcriptional changes in heart or renal stress models, including pathways linked to fibrosis and stress signaling.
• Systems-Level Transcriptomics: Using microarray or RNA-seq to analyze multi-organ gene-expression shifts following Vilon exposure, focusing on extracellular matrix, inflammation, and stress-response networks.

Note: Experimental parameters (such as concentration, exposure time, and model selection) must be determined by researchers based on study goals, prior research, and institutional oversight. No dosing or clinical guidance is provided or implied.

Handling and Storage Recommendations

• Store sealed at −20 °C, protected from light, tightly sealed.
• For short-term storage, keep at 2–8 °C.
• Allow the vial to reach room temperature before opening to reduce condensation.
• If preparing solutions, use aseptic technique and follow your lab’s SOPs for aliquoting, stability controls, and disposal.

Research Use Only Notice

This product is intended for laboratory research use only and is not approved for human or veterinary use. It is not intended for diagnostic, therapeutic, or clinical applications. Any reference to biological activity or potential effects is based solely on preclinical or in‑vitro findings and should not be interpreted as validated clinical outcomes. Researchers are responsible for ensuring proper handling, storage, and disposal in accordance with institutional, federal, and international guidelines.

References

1. Lezhava T, Khavison V, Monaselidze J, et al. Bioregulator Vilon-Induced Reactivation of Chromatin in Cultured Lymphocytes from Old People. Biogerontology. 2004;5(2):73-79. doi:10.1023/b:bgen.0000025070.90330.7f
2. Kazakova TB, Barabanova SV, Khavinson VKh, et al. In vitro effect of short peptides on expression of interleukin-2 gene in splenocytes. Bulletin of Experimental Biology and Medicine. 2002;133(6):614-616. doi:10.1023/a:1020210615148
3. Sevostianova NN, Linkova NS, Polyakova VO, et al. Immunomodulating effects of vilon and its analogue in the culture of human and animal thymus cells. Bulletin of Experimental Biology and Medicine. 2013;154(4):562-565. doi:10.1007/s10517-013-2000-0
4. Lezhava T, Monaselidze J, Kadotani T, Dvalishvili N, Buadze T. Anti-aging peptide bioregulators induce reactivation of chromatin. PubMed. Published April 1, 2006. https://pubmed.ncbi.nlm.nih.gov/16705247/
5. Anisimov SV, Bokheler KR, Khavinson VKh, Anisimov VN. Studies of the Effects of Vilon and Epithalon on Gene Expression in Mouse Heart using DNA-Microarray Technology. Bulletin of Experimental Biology and Medicine. 2002;133(3):293-299. doi:10.1023/a:1015859322630
6. Gavrisheva NA, Malinin VV, Ses TP, Kozlov KL, Panchenko AV, Titkov AYu. Effect of peptide Vilon on the content of transforming growth factor-β and permeability of microvessels during experimental chronic renal failure. Bulletin of Experimental Biology and Medicine. 2005;139(1):24-26. doi:10.1007/s10517-005-0202-9