SS-31 (50mg)

What is SS-31 (50 mg)?

SS-31 (50 mg), also known as Elamipretide or MTP-131, is a synthetic tetrapeptide designed for advanced research into mitochondrial function and cellular energy production. It selectively associates with the inner mitochondrial membrane, specifically interacting with cardiolipin, a phospholipid essential for mitochondrial integrity and efficient energy production.

The peptide’s structure includes both cationic and aromatic residues, allowing stable interactions with the negatively charged mitochondrial membranes. These features make SS-31 a valuable tool for investigating mitochondrial respiration, oxidative phosphorylation, membrane stability, and redox balance in laboratory settings.

This product is strictly for laboratory research use and is not intended for clinical or therapeutic applications.

Product Specifications

• CAS Number: 736992-21-5
• Synonyms: MTP-131, Elamipretide
• Peptide Sequence: D-Arg-dimethyl-Tyr-Lys-Phe-NH₂
• Molecular Formula: C₃₂H₄₉N₉O₅
• Molecular Weight: 639.8 g/mol
• Purity: ≥98% (verified by HPLC and mass spectrometry), lot-specific COA
• Storage Conditions: Store at −20 °C, protected from light and moisture. Let the vial reach room temperature before opening; limit temperature cycling.
• Intended Use: For Laboratory Research Use Only

Key Characteristics of SS-31 (50 mg)

• Mitochondrial-Targeted Peptide: SS-31 is designed to selectively interact with the inner mitochondrial membrane, making it an essential tool for mitochondrial research.
• Defined Tetrapeptide Structure: The peptide’s structure enables consistent and reproducible results across different experimental systems.
• Membrane-Active Properties: The combination of positively charged and aromatic residues supports stable membrane association, helping researchers study mitochondrial dynamics.
• Lyophilized Format: The freeze-dried (lyophilized) form preserves the peptide’s integrity before reconstitution.
• Widely Used in Metabolic Research: Frequently incorporated into studies investigating mitochondrial function, oxidative stress, and bioenergetics.
• Research-Focused Formulation: Ideal for in vitro experiments and preclinical models evaluating cellular bioenergetics and mitochondrial health.

How SS-31 (50 mg) Supports Research

SS-31 (50 mg) is used to study mitochondrial function and bioenergetic efficiency. Because mitochondria are crucial for ATP production, ROS generation, and metabolic signaling, SS-31 enables researchers to explore how targeted interactions with mitochondrial membranes influence cellular stability and energy dynamics.

In experimental systems, researchers use SS-31 to investigate its interaction with cardiolipin, helping to understand mitochondrial membrane organization, electron transport chain activity, and redox regulation. Its defined structure enables precise evaluation in cell-based assays and preclinical models focused on mitochondrial stress and dysfunction.

Research Applications & Usage Information

SS-31 (50 mg) is typically used in controlled laboratory research for:

• Mitochondrial Respiration and ATP Production Assays: To measure mitochondrial efficiency in energy production.
• Electron Transport Chain and Oxidative Phosphorylation Studies: Used to explore mitochondrial function and ATP synthesis.
• Reactive Oxygen Species (ROS) and Redox Balance Research: Investigates how SS-31 influences oxidative stress and cellular redox signaling.
• Mitochondrial Membrane Integrity Investigations: To assess the stability and health of mitochondrial membranes under various conditions.
• Cellular Bioenergetics and Metabolic Signaling Analysis: Studies involving mitochondrial energy production and metabolic pathways.
• Preclinical Models of Mitochondrial Stress or Dysfunction: Examining mitochondrial responses in stress-related models.
• Comparative Studies of Mitochondria-Targeted Peptides: Comparing the effects of SS-31 with other peptides targeting mitochondrial membranes.

All applications are strictly for laboratory research environments.

Handling and Storage Recommendations

• Store at 2–8 °C (≤–20 °C for long-term storage), protected from light.
• Keep vials tightly sealed and protected from moisture until use.
• Allow the vial to equilibrate to room temperature before opening to minimize exposure to condensation.
• After reconstitution, store prepared solutions at 2–8 °C, as per laboratory protocol.
• Avoid repeated freeze-thaw cycles to maintain peptide stability.
• Follow appropriate sterile handling procedures during preparation and experimentation.

Research Use Only Notice

This product is intended for laboratory research use only and is not approved for human or veterinary use. It is not intended for diagnostic, therapeutic, or clinical applications. Any reference to biological activity or potential effects is based solely on preclinical or in-vitro findings and should not be interpreted as validated clinical outcomes. Researchers are responsible for ensuring proper handling, storage, and disposal in accordance with institutional, federal, and international guidelines.

References

1. PubChem CID 11764719. National Center for Biotechnology Information. https://pubchem.ncbi.nlm.nih.gov/compound/Elamipretide
2. Elamipretide: Uses, interactions, mechanism of action. DrugBank. https://go.drugbank.com/drugs/DB11981
3. Birk AV, Liu S, Soong Y, et al. The mitochondrial-targeted compound SS-31 re-energizes ischemic mitochondria by interacting with cardiolipin. J Am Soc Nephrol. 2013;24(8):1250-1261. doi:10.1681/ASN.2012121216
4. Tse BC, Dvoriantchikova G, Tao W, et al. Mitochondrial targeted therapy with elamipretide (MTP-131) as an adjunct to tumor necrosis factor inhibition for traumatic optic neuropathy in the acute setting. Exp Eye Res. 2020;199:108178. doi:10.1016/j.exer.2020.108178
5. Brown DA, Hale SL, Baines CP, et al. Reduction of early reperfusion injury with the mitochondria-targeting peptide bendavia. J Cardiovasc Pharmacol Ther. 2014;19(1):121-132. doi:10.1177/1074248413508003
6. Li M, Kong D, Meng L, Wang Z, Bai Z, Wu G. Discovery of novel SS-31 (d-Arg-dimethylTyr-Lys-Phe-NH2) derivatives as potent agents to ameliorate inflammation and increase mitochondrial ATP synthesis. RSC Adv. 2024;14(41):29789-29799. Published 2024 Sep 18. doi:10.1039/d4ra05517a