Snap-8 (10mg)

What is SNAP-8 (10 mg)?

SNAP-8 (10 mg) is a synthetic acetylated octapeptide (8 amino acids) with the sequence Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH₂. It was designed to mimic a segment of SNAP-25, a protein involved in processes like neurotransmitter release through the SNARE machinery.

SNAP-8 is frequently grouped with aesthetic and cosmetic peptides due to its role in skin-related and topical research. It is studied in models focused on neuromodulation and cellular release (exocytosis) in peripheral tissues.

In laboratory research, SNAP-8 (also known as Acetyl Octapeptide-3) is used to explore how peptide-based inhibitors can interact with SNARE complexes and influence processes like membrane fusion and neurotransmitter release.

Product Specifications

• CAS Number: 868844-74-0
• Peptide Sequence: Acetyl-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH₂
• Chemical Formula: C₄₁H₇₀N₁₆O₁₆S
• Molecular Weight: 1075.16 g/mol
• Purity: Research-grade peptide, typically ≥98–99% by HPLC. Identity and purity are verified using HPLC and mass spectrometry, with batch-specific results provided in a certificate of analysis (COA).
• Packaging Format: 10 mg lyophilized (freeze-dried) SNAP-8 in a sealed vial, suitable for preparing controlled stocks for topical formulation research, cell-based assays, or biochemical studies.
• Storage Conditions: Store at −20 °C, protected from light and moisture. Avoid repeated temperature cycling to help preserve peptide integrity.
• Intended Use: For laboratory research use only; not for human or veterinary use.

Key Characteristics of SNAP-8 (10 mg)

• SNAP-25 Mimetic Peptide: SNAP-8 is an N-acetylated and C-amidated octapeptide engineered to resemble a functional region of SNAP-25, a key component of the SNARE complex involved in exocytosis and vesicle fusion.
• Interference with SNARE Complex Formation: In biochemical and cell-based assays, SNAP-8 is studied for its ability to compete with SNAP-25 interactions, affecting the efficiency of SNARE complex assembly and vesicle fusion.
• Cosmetic and Topical Research Relevance: SNAP-8 is frequently evaluated in topical research and skin models that track changes in release processes and contraction-related effects. It is often compared to shorter mimetic peptides, such as acetyl hexapeptide-8 (Argireline), in studies examining relative activity and persistence.
• Stability-Focused Modifications: The acetylated N-terminus and amidated C-terminus provide greater resistance to enzymatic breakdown and more consistent behavior in assays, including cell-free systems and topical delivery research.
• Research-Ready Analytical Support: Lots are typically supplied with HPLC chromatograms and MS confirmation, supporting repeatability in studies on SNARE-related inhibition and membrane biology.

How SNAP-8 (10 mg) Supports Research

SNAP-8 (10 mg) is used in research as a targeted tool to study the vesicle fusion pathway. By acting as a competitive peptide that can disrupt SNAP-25-associated interactions, it enables researchers to measure how partial interference influences:

• SNARE “zippering” and complex assembly
• Fusion probability and kinetics
• Downstream release events in both cell-free systems and live-cell models

Within cosmetic and aesthetic research, SNAP-8 is applied to study neuromodulation in skin-relevant systems, including release-linked and contraction-related readouts. In broader cell biology and neuroscience research, it also serves as a tool compound for investigating SNARE mechanisms.

Research Applications & Usage Information

• SNARE Assembly and Fusion Kinetics: Used in purified-protein reconstitution assays to measure effects on SNARE complex formation, assembly speed, and fusion readouts (e.g., lipid-mixing and content-mixing assays).
• Exocytosis Modulation in Cell Models: Incorporated into neurosecretory or endocrine cell systems to evaluate graded effects on vesicle release, neurotransmitter-related readouts, or hormone secretion under controlled stimulation.
• Topical Neuromodulation Studies: Applied in skin organoid and contraction-linked assays to assess changes in release signaling and muscle dynamics, often comparing SNAP-8 with Argireline for relative activity in the same model.
• Structure–Function Mapping: Used in competitive binding designs to identify which regions of SNAP-25 contribute most to SNARE complex interfaces, including testing interactions with syntaxin and synaptobrevin.
• Comparative SNARE-Modulator Research: Studied alongside other SNARE-active agents to compare competitive interference versus mechanisms that alter SNARE function through other pathways.

Note: Experimental parameters (e.g., concentration, incubation time, and model selection) must be set by qualified investigators in accordance with applicable protocols and regulatory requirements. No dosing or clinical guidance is provided or implied.

Handling and Storage Recommendations

• Store the sealed vial at −20 °C, protected from light and moisture.
• Let the vial reach room temperature before opening to reduce condensation.
• Use standard laboratory PPE and follow good laboratory practices for bioactive peptides.
• If preparing research solutions, follow your institution’s validated procedures for solvent choice, labeling, stability controls, and disposal.
• Discard prepared materials if visible changes occur (e.g., precipitation or discoloration), per internal stability criteria.

Research Use Only Notice

This product is intended for laboratory research use only and is not approved for human or veterinary use. It is not intended for diagnostic, therapeutic, or clinical applications. Any reference to biological activity or potential effects is based solely on preclinical or in‑vitro findings and should not be interpreted as validated clinical outcomes. Researchers are responsible for ensuring proper handling, storage, and disposal in accordance with institutional, federal, and international guidelines.

References

1. Tafoya LCR, Mameli M, Miyashita T, Guzowski JF, Valenzuela CF, Wilson MC. Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons. Journal of Neuroscience. 2006;26(30):7826-7838. doi:10.1523/jneurosci.1866-06.2006
2. Khvotchev M, Soloviev M. SNARE modulators and SNARE mimetic peptides. Biomolecules. 2022;12(12):1779. doi:10.3390/biom12121779
3. Blanes‐Mira C, Clemente J, Jodas G, et al. A synthetic hexapeptide (Argireline) with antiwrinkle activity. International Journal of Cosmetic Science. 2002;24(5):303-310. doi:10.1046/j.1467-2494.2002.00153.x
4. Fasshauer D, Margittai M. A transient n-terminal interaction of SNAP-25 and Syntaxin nucleates SNARE assembly. Journal of Biological Chemistry. 2004;279(9):7613-7621. doi:10.1074/jbc.m312064200
5. Errante F, Ledwoń P, Latajka R, Rovero P, Papini AM. Cosmeceutical peptides in the framework of sustainable Wellness economy. Frontiers in Chemistry. 2020;8:572923. doi:10.3389/fchem.2020.572923