Recent advances in aesthetic medicine highlight the critical role of precise dilution when using hyaluronidase. A guidance document by King, Convery, and Davies (2017) revealed that improper dilution can dramatically compromise the enzyme’s ability to break down hyaluronic acid fillers—potentially increasing the risk of adverse reactions. Proper preparation is key to maximizing both safety and effectiveness.
Among the most widely used hyaluronidase formulations is Liporase, a trusted enzyme in aesthetic practice. To ensure consistent results and minimize complications, Liporase must be accurately diluted before clinical use. Getting the balance right is essential—not only for performance but also for patient safety.
In this article, we’ll walk through a step-by-step guide on how to properly dilute Liporase, sharing best practices and expert insights to support safe and successful outcomes in every treatment session.
Key Takeaways
- Liporase is a purified hyaluronidase enzyme used in aesthetic medicine to dissolve hyaluronic acid-based fillers and correct cosmetic complications with precision.
- Proper dilution is essential for maintaining enzyme stability and achieving safe, consistent results. The standard dilution involves mixing 1 vial of Liporase with 3 mL of 0.9% NaCl saline.
- Sterile technique and accurate measurement are critical throughout the dilution process to minimize the risk of contamination and ensure effective filler breakdown.
- Slow mixing and careful injection preserve the enzyme’s integrity and help avoid adverse reactions such as nodules or tissue damage.
- Common mistakes, including over-shaking, incorrect dilution ratios, or non-sterile tools, can compromise treatment outcomes and patient safety.
- Clinicians must follow manufacturer guidelines and stay updated on best practices to deliver safe, effective, and personalized aesthetic corrections.
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Understanding Liporase: Composition and Uses
Many individuals may ask medical professionals, “What is Liporase?” In aesthetic medicine, Liporase refers to a purified hyaluronidase enzyme commonly used to dissolve hyaluronic acid-based dermal fillers or in some cases, assist fat-dissolving injections. This enzyme has proven effective for correcting aesthetic enhancements, allowing clinicians to make subtle or significant modifications with precision.
Manufactured by Daehan Nupharm in South Korea, Liporase breaks down the glycosidic bonds in synthetic hyaluronic acid. What sets Liporase apart is its ability to do this without damaging the body’s naturally occurring hyaluronic acid, thanks to its refined formulation. This targeted action supports accurate aesthetic corrections and minimizes the risk of adverse reactions, making it ideal for medical professionals seeking reliable, controlled adjustments.
Clinicians often turn to Liporase to correct filler misplacements, reverse overcorrections, or treat complications such as vascular occlusions. Its high efficacy and established safety profile have made it a trusted product for practitioners worldwide, offering minimally invasive, patient-specific correction without requiring surgical intervention.
Required Materials for Diluting Liporase
Proper dilution is essential to activate Liporase effectively and ensure optimal results. Having the right tools and understanding their purpose are critical for maintaining sterility and precision during preparation. Below are the essential materials every practitioner should have on hand:
- Liporase Vial: The core enzyme for filler dissolution.
- Sterile 0.9% Sodium Chloride (NaCl) Saline Solution: Ensures consistent dilution while maintaining a sterile medium.
- Sterile Mixing Container and Syringe: Necessary for accurate measurements, mixing, and drawing up the solution.
- Aseptic Supplies: Including sterile gloves, alcohol swabs, and clean work surfaces to prevent contamination.
Gathering these materials in advance ensures a streamlined workflow, enabling medical professionals to maintain control over enzyme concentration and treatment precision.
Step-by-Step Instructions for Liporase Dilution
Maintaining enzyme integrity begins with a proper dilution technique. The following clinical protocol is widely accepted and reflects current best practices in aesthetic medicine:
- Prepare the Vial: In a sterile environment, inject 1 mL of 0.9% NaCl saline into the Liporase vial using a sterile syringe.
- Dissolve the Powder: Rotate or gently swirl the vial until the powder dissolves completely. Avoid shaking to prevent enzyme degradation.
- Complete the Dilution: Add 2 more mL of saline, bringing the total volume to 3 mL.
- Administer the Solution: Inject directly into the target area, matching the volume of Liporase solution to the filler quantity using a one-to-one ratio when applicable.
Following these steps closely ensures stable enzymatic activity, effective breakdown of hyaluronic acid, and minimal complications during correction.
Best Practices for Liporase Administration
Consistently successful Liporase applications rely on strict procedural adherence. Key recommendations for safe and effective administration include:
- Sterile Reconstitution: Always reconstitute Liporase using 0.9% NaCl saline in a sterile environment.
- Correct Dilution Ratio: For most aesthetic uses, one vial (typically 1,500 IU) should be diluted with 3 mL of saline. This dilution provides a reliable concentration for common corrections.
- Gentle Injection Technique: Inject the product slowly while closely monitoring for tissue response and patient comfort.
Medical professionals must remain updated on evolving clinical recommendations and continually practice safe handling. This ensures consistency in enzyme activity, reduces risks, and supports long-term patient satisfaction.
Common Mistakes to Avoid When Diluting Liporase
Even skilled professionals can make errors during preparation. Some of the most common dilution errors include:
- Incorrect Saline Volume: Misjudging the dilution ratio alters the enzyme’s strength and may compromise results.
- Aggressive Mixing: Shaking the vial vigorously can denature the enzyme, reducing its effectiveness.
- Using Non-Sterile Tools: Any breach in sterile technique increases the risk of infection or adverse reactions.
- Ignoring Manufacturer Guidelines: Skipping detailed instructions may result in underperformance or unexpected side effects.
By recognizing these pitfalls, professionals can improve outcomes and ensure high standards of patient safety in every procedure.
Conclusion
Diluting Liporase properly is crucial for achieving safe and effective aesthetic results. By following the recommended steps and using sterile technique, practitioners can harness the full potential of this versatile hyaluronidase enzyme. Attention to detail—such as precise dilution ratios, careful handling, and avoiding common mistakes—ensures both enzyme stability and clinical success.
Ultimately, staying informed and up-to-date with evidence-based protocols will allow aesthetic professionals to offer customized, corrective treatments that preserve natural beauty and build patient trust.
FAQs
1. Why is precise dilution important when using Liporase?
Precise dilution is crucial because incorrect dilution can reduce the effectiveness of Liporase in breaking down hyaluronic acid fillers and may increase the risk of adverse reactions. Proper dilution ensures optimal performance and minimizes unintended side effects.
2. What materials are needed for the dilution of Liporase?
To dilute Liporase, you need a Liporase vial, sterile 0.9% NaCl saline solution, a sterile mixing container, a syringe, and aseptic supplies like gloves and alcohol wipes to maintain a sterile environment.
3. What are the key steps in the dilution process for Liporase?
The key steps involve adding 1 mL of sterile saline to the Liporase vial, gently mixing until fully dissolved, adding 2 mL of saline for a total of 3 mL, and then administering the solution into the target area based on the filler volume.
References
- King, M., Convery, C., & Davies, E. (2017). The Use of Hyaluronidase in Aesthetic Practice Title The Use of Hyaluronidase in Aesthetic Practice The Use of Hyaluronidase in Aesthetic Practice Background. https://www.baman.org.uk/content/large/documents/members_documents/complications_guidance/acegrouphyaluronidasev2.4.pdf
- Daehan Nupharm. (n.d.). LIPOLASE Inj. Dhnp.co.kr. Retrieved April 23, 2025, from https://www.dhnp.co.kr/en/products/product_view.aspx?menu=0101&prod_cd=%20A-00013&category=01
